Journal: Arhiv za bioloske nauke

Article Title: Comparative study of the differentiation potential of rat bone marrow mesenchymal stem cells and rat muscle-derived stem cells

doi: 10.2298/abs1304307i

Figure Lengend Snippet: Fig. 1 Analysis of osteogenic lineage markers of in vitro differentiated rat bone marrow MSCs and MDSCs at 10 days after induced osteogenic stimulation using Osteo Diff Medium. Von Kossa silver nitrate staining revealed the presence of hydroxyapatite crystals in the extracellular matrix of differentiated cells, stained in black for bone marrow MSCs (a) and MDSCs (b); magnification 100x. Immu- nohistochemical analysis for amelogenin expression of differentiated cells stained in red for bone marrow MSCs (c) and MDSCs (d), at 10 days after induced osteogenic stimulation; magnification 400x.

Article Snippet: Amelogenin extracellular matrix localization Immunohistochemical analysis of amelogenin presence was performed using an amelogenin antibody (Santa Cruz Biotechnology, INC, Santa Cruz, USA).

Techniques: In Vitro, Staining, Expressing

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: Expression of ECM1 in HCC and normal liver tissues

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: Correlation between the expression of ECM1 in 120 HCC tissues and their clinicopathological characteristics

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: The correlation between ECM1 and Vimentin expression in HCC tissues

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: a Kaplan-Meier analysis of overall survival curve of HCC patients according to ECM1 expression. The HCC patients with ECM1-positive showed significantly shorter overall survival survival rates than those with ECM1-negative. b Kaplan-Meier analysis of disease-free survival curve of HCC patients according to ECM1 expression. The expression of ECM1 is associated with decreased disease-free survival rate

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: Univariate survival analysis of OS and DFS in 120 patients with HCC

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: Multivariate survival analysis of OS and DFS in 120 patients with HCC

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: a Representative pictures of ECM1 staining in Bel-7402 cells, ECM1 mainly expressed in the cytoplasm of cells. b Representative pictures of ECM1 staining in ECM1-Bel-7402 cells. The ECM1 level of Bel-7402 cells was significantly increased detected by immunohistochemical staining. Original magnification at ×200

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Staining, Immunohistochemical staining

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: a Changes of ECM1 and EMT-related protein (E-cadherin and Vimentin) expression in Bel-7402 cells were detected by Western blot after ECM1 transfection. The increased expression of ECM1 could lead to the up-regulated expression of Vimentin protein and down-regulated expression of E-cadherin protein in Bel-7402 cells. b The relative quantity of three protein expressed levels in cells with significant difference (ECM1: t = 12.909, P < 0.01; Vimentin; t = 10.564, P < 0.01; E-cadherin: t = −8.558, P = 0.002). Relative quantity of three protein expressed levels in cells. Data are mean ± SD of three replicates. ( P < 0.05)

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Expressing, Western Blot, Transfection

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: a Effect of ECM1 on the migration capacity of Bel-7402 cell by the wound healing assay at 24, 48, and 72 h (×40). b Statistics of analysis of migration rate date in Bel-7402 and ECM1-Bel-7402 cells. The wound healing assay revealed that the migration rates of the cells in the Bel-7402 group infected with pEGFP-N2-ECM1 were significantly increased compared with those without transfected group ( F = 110.592, P < 0.01). Data are mean ± SD of three replicates. ( P < 0.05)

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Migration, Wound Healing Assay, Infection, Transfection

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: a , b Effect of ECM1 on the invasion capacity of Bel-7402 and ECM1-Bel-7402 cells by invasion assay (×200). c Statistics of analysis of cells per field in Bel-7402 and ECM1-Bel-7402 cells at 48 h. Compared with the control group, after the up-regulated expression of ECM1, the number of invading Bel-7402 cells was significantly increased (89.3 ± 2.5 cells/well vs 28.7 ± 2.5 cells/well) ( t = 29.524, P < 0.01). Data are mean ± SD of three replicates ( P < 0.05)

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Invasion Assay, Control, Expressing

Journal: The international journal of biochemistry & cell biology

Article Title: ECM2, a prognostic biomarker for lower grade glioma, serves as a potential novel target for immunotherapy.

doi: 10.1016/j.biocel.2023.106409

Figure Lengend Snippet: Fig. 1. The expression pattern of ECM2 in LGG and normal brain tissues. (A-B) The expression changes of ECM2 in multiple tumors based on the GEPIA database, including LGG and GBM. Up-regulation in red and down-regulation in green. (C) The expression of ECM2 in LGG based on GSE12657 dataset. (D-E) The mRNA and protein levels of ECM2 based on laboratory samples. * P < 0.05, * * P < 0.01.

Article Snippet: Next, the specific antibody targeting ECM2 (Proteintech, China) was utilized to treat brain samples overnight at 4 °C.

Techniques: Expressing

Journal: The international journal of biochemistry & cell biology

Article Title: ECM2, a prognostic biomarker for lower grade glioma, serves as a potential novel target for immunotherapy.

doi: 10.1016/j.biocel.2023.106409

Figure Lengend Snippet: Fig. 2. The correlation between ECM2 expression with clinical and molecular features of LGG. TCGA database: (A) WHO Grade, (B) Histology, (C) Chemo status, (D) IDH mutation status; CGGA database: (E) PRS type, (F) Histology, (G) IDH mutation, (H) 1p19q codeletion, (I) MGMTp methylation.

Article Snippet: Next, the specific antibody targeting ECM2 (Proteintech, China) was utilized to treat brain samples overnight at 4 °C.

Techniques: Expressing, Mutagenesis, Methylation

Journal: The international journal of biochemistry & cell biology

Article Title: ECM2, a prognostic biomarker for lower grade glioma, serves as a potential novel target for immunotherapy.

doi: 10.1016/j.biocel.2023.106409

Figure Lengend Snippet: Fig. 3. The relationship between ECM2 expression and overall survival of LGG patients with different WHO grades. TCGA database: (A) Grade II and III, (B) Grade II, (C) Grade III; CGGA database: (D) Grade II and III, (E) Grade II, (F) Grade III.

Article Snippet: Next, the specific antibody targeting ECM2 (Proteintech, China) was utilized to treat brain samples overnight at 4 °C.

Techniques: Expressing

Journal: The international journal of biochemistry & cell biology

Article Title: ECM2, a prognostic biomarker for lower grade glioma, serves as a potential novel target for immunotherapy.

doi: 10.1016/j.biocel.2023.106409

Figure Lengend Snippet: Fig. 5. The prognostic value of ECM2. (A-B) Univariate analysis on clinical characters of LGG based on TCGA and CGGA database. (C-D) Multivariate analysis on clinical characters of LGG based on TCGA and CGGA database. (E) The meta-analysis of ECM2 prognostic value based on TCGA database, CGGA database and GSE43378 dataset.

Article Snippet: Next, the specific antibody targeting ECM2 (Proteintech, China) was utilized to treat brain samples overnight at 4 °C.

Techniques:

Journal: The international journal of biochemistry & cell biology

Article Title: ECM2, a prognostic biomarker for lower grade glioma, serves as a potential novel target for immunotherapy.

doi: 10.1016/j.biocel.2023.106409

Figure Lengend Snippet: Fig. 6. The co-expression analysis and GSEA analysis on ECM2. (A-B) The most positively or negatively correlated genes of ECM2. (C-D) The results of signaling pathway enrichment of ECM2 based on TCGA and CGGA database.

Article Snippet: Next, the specific antibody targeting ECM2 (Proteintech, China) was utilized to treat brain samples overnight at 4 °C.

Techniques: Expressing

Journal: The international journal of biochemistry & cell biology

Article Title: ECM2, a prognostic biomarker for lower grade glioma, serves as a potential novel target for immunotherapy.

doi: 10.1016/j.biocel.2023.106409

Figure Lengend Snippet: Fig. 7. The correlation of ECM2 expression and the infiltration of immune cells. (A) The relationship between ECM2 expression with tumor purity and six immune cells. (B) The relationship between the infiltration of immune cells and overall survival of LGG patients. (C) The abundance of immune cells and tumor cells based on ESTIMATE analysis. (D-J) The molecular markers of each immune cell with the highest correlation with ECM2.

Article Snippet: Next, the specific antibody targeting ECM2 (Proteintech, China) was utilized to treat brain samples overnight at 4 °C.

Techniques: Expressing

Journal: The international journal of biochemistry & cell biology

Article Title: ECM2, a prognostic biomarker for lower grade glioma, serves as a potential novel target for immunotherapy.

doi: 10.1016/j.biocel.2023.106409

Figure Lengend Snippet: Fig. 8. The correlation between the expression of ECM2 and immune checkpoints. (A-H) The correlation of ECM2 and HAVCR2, CD274, CD96, PDCD1, CD276, IDO1, CTLA4, KLRB1. (I-J) The protein expression of CD163 and PD-L1 in LGG and normal brain tissues. * P < 0.05, * ** * P < 0.0001.

Article Snippet: Next, the specific antibody targeting ECM2 (Proteintech, China) was utilized to treat brain samples overnight at 4 °C.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Hyaluronic acid−CD44 signaling from decidual stromal cells orchestrates dNK1 differentiation and immune tolerance in early pregnancy

doi: 10.3389/fimmu.2026.1777567

Figure Lengend Snippet: HA/CD44 axis promotes NK92MI tissue residency. (A) Analysis of HAS2 expression levels across different cell subpopulations in normal decidual tissue based on scRNA-seq data ( n = 11). (B) The expression of HAS2 in human decidual tissue (scale bars, 100 μm) by immunohistochemistry ( n = 10 per group). (C) Confirmation of HAS2KD in DSCs by western blot ( n = 3 per group). (D) HA concentration in DSC supernatant ( n = 6 per group). (E) The residency of NK92MI cells in DSCs (scale bars, 50 μm) after co-culture with HAS2KD DSCs ( n = 9 per group). (F, G) The expression of CD49a and adhesion molecules ( ICAM-1, VCAM-1 , and ITGAX ) in NK92MI cells after co-culture with HAS2KD DSCs ( n = 6 per group). (H) CD49a expression in NK92MI cells co−cultured with control DSCs or HAS2KD DSCs, with or without exogenous HMW−HA ( n = 6 per group). (I) CD49a expression in NK92MI cells from DSC-NK co-cultures treated with exogenous HMW-HA after CD44 blocking ( n = 6 per group). (J, K) The expression of CD49a and adhesion molecules ( ICAM-1, VCAM-1 , and ITGAX ) in NK92MI cells co−cultured with control DSCs or HAS2KD DSCs, with or without CD44−blocking antibody. Data are expressed as mean ± SD; * P < 0.05; ** P < 0.01; ns, not significant. EP, early pregnancy; SA, spontaneous abortion.

Article Snippet: To determine the signaling pathways involved, we used an anti-CD44 blocking antibody (30 μg/mL; BioxCell, BE0262) to block CD44, and IWP-2 (50 μM; MedChemExpress, HY-13912) to inhibit the canonical Wnt pathway.

Techniques: Expressing, Immunohistochemistry, Western Blot, Concentration Assay, Co-Culture Assay, Cell Culture, Control, Blocking Assay

Journal: Frontiers in Immunology

Article Title: Hyaluronic acid−CD44 signaling from decidual stromal cells orchestrates dNK1 differentiation and immune tolerance in early pregnancy

doi: 10.3389/fimmu.2026.1777567

Figure Lengend Snippet: DSCs ameliorate NK92MI cell cytotoxicity by modulating CD44 subpopulations via the HA/CD44 axis. (A) The proportion of the CD44 high subpopulation in NK92MI cells after co-culture with DSCs ( n = 6 per group). (B) The expression of cytokines (GZMB, TNF-α, IFN-γ, TGF-β1 and IL-10) in NK92MI cells detected by flow cytometry after co-culture with DSCs ( n = 5 per group). (C) The viability of K562 cells determined after exposure to NK cells previously co-cultured with DSCs ( n = 6 per group). (D, E) The proportion of the CD44 high subpopulation and the expression of effector molecules (GZMB, TNF−α, IFN−γ, TGF−β1 and IL−10) in NK92MI cells co−cultured with control DSCs or HAS2KD DSCs, with or without CD44−blocking antibody ( n = 6 per group). (F, G) The proportion of the CD44 high subpopulation in NK92MI cells co−cultured with control DSCs, HAS2KD DSCs, or DSCs plus CD44−blocking antibody after the addition of exogenous HMW−HA ( n = 5 per group). Data are expressed as mean ± SD; ** P < 0.01; ns, not significant.

Article Snippet: To determine the signaling pathways involved, we used an anti-CD44 blocking antibody (30 μg/mL; BioxCell, BE0262) to block CD44, and IWP-2 (50 μM; MedChemExpress, HY-13912) to inhibit the canonical Wnt pathway.

Techniques: Co-Culture Assay, Expressing, Flow Cytometry, Cell Culture, Control, Blocking Assay

Journal: Frontiers in Immunology

Article Title: Hyaluronic acid−CD44 signaling from decidual stromal cells orchestrates dNK1 differentiation and immune tolerance in early pregnancy

doi: 10.3389/fimmu.2026.1777567

Figure Lengend Snippet: DSCs ameliorate NK cell cytotoxicity by regulating the proportion of CD44 high and CD44 low subpopulations via the HA/CD44 axis. (A) The proportion of dNK1, dNK2, and dNK3 subpopulations in dNK cells from individuals with normal pregnancies and SA ( n = 19 per group). (B) The viability of K562 cells after co-culture with total dNK cells from individuals of normal pregnancies and SA ( n = 9 per group). (C) UMAP plot of scRNA-seq data from normal decidual tissues showing elevated CD44 expression in the dNK2 and dNK3 subpopulation ( n = 11). (D) The proportion of the CD44 high subpopulation within the three subpopulations in individuals with normal pregnancies ( n = 6). (E) The proportion of the CD44 high subpopulation in total dNK cells and the three subpopulations between individuals from normal pregnancies and SA ( n = 6 per group). Data are expressed as mean ± SD; ** P < 0.01; ns, not significant. EP, early pregnancy; SA, spontaneous abortion.

Article Snippet: To determine the signaling pathways involved, we used an anti-CD44 blocking antibody (30 μg/mL; BioxCell, BE0262) to block CD44, and IWP-2 (50 μM; MedChemExpress, HY-13912) to inhibit the canonical Wnt pathway.

Techniques: Co-Culture Assay, Expressing

Journal: Frontiers in Immunology

Article Title: Hyaluronic acid−CD44 signaling from decidual stromal cells orchestrates dNK1 differentiation and immune tolerance in early pregnancy

doi: 10.3389/fimmu.2026.1777567

Figure Lengend Snippet: HA-induced activation of the canonical Wnt pathway upregulates FOSL2 to expand the dNK1-like subpopulation. (A) Analysis of transcriptional activity and expression levels of transcription factors in three dNK subsets using scRNA-seq data from normal decidual tissue ( n = 11). (B) Transcriptional activity and expression levels of FOSL2 in decidual tissues from normal pregnancy ( n = 5) and RSA groups ( n = 3). (C) Changes in NK92MI cells of the five transcription factors ( STAT3, MAFB, HES1, FOSL2, ETV5 ) characterized by high transcriptional activity and expression in the dNK1 subset following HMW-HA treatment, as determined by RT−qPCR ( n = 6 per group). (D) FOSL2 expression in NK92MI cells following HMW-HA treatment, assessed by WB ( n = 3 per group). (E) Wnt1 and β-catenin protein expression in NK92MI cells treated with or without HMW-HA in the presence or absence of CD44 blockade ( n = 3 per group). (F) FOSL2 expression in HA−treated NK92MI cells after addition of CD44−blocking antibody or the Wnt pathway inhibitor IWP−2 to the culture system ( n = 3 per group). Data are expressed as mean ± SD; * P < 0.05; ** P < 0.01; ns, not significant. EP, early pregnancy; SA, spontaneous abortion; RSA, recurrent spontaneous abortion.

Article Snippet: To determine the signaling pathways involved, we used an anti-CD44 blocking antibody (30 μg/mL; BioxCell, BE0262) to block CD44, and IWP-2 (50 μM; MedChemExpress, HY-13912) to inhibit the canonical Wnt pathway.

Techniques: Activation Assay, Activity Assay, Expressing, Quantitative RT-PCR, Blocking Assay